The Journal of Maltese Science – "Perfect Outcast" Edition

[Hunor]

The Journal of Maltese Science - "Perfect Outcast" Edition
05.01.829

Crystal Cardamine

Prof. P. Ferrari et al

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Introduction

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In view of the new super cardamine-type plant reported in our previous article, further experiments were performed at the Galicia Research Station. Given the protein structure of the genetically modified Cardamine, the following results were encountered during bio-phytic and biochemical testing of additional liquid and solid forms.

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Selected Principles

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Crystallization is the formation of an ordered solid phase. The key word here is ‘ordered’—a molecule needs to adopt a structured repeating unit to form a stable solid phase that can be considered crystalline. Nucleation and growth are both driven by supersaturation—the difference between the solubility and the concentration of the solution — which needs to be directed to enable control over the outcome of the crystallization process.

Supersaturation relates to two of the main transfer processes that occur in the crystallization vessel: the mass transfer and the heat transfer. Crystals may have different faces that can grow at different rates and if this growth can be controlled, the size of the particles can be regulated, as well as limiting any impurity inclusion from the crystals being grown.

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Material and Methods

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Cocrystallization of RNA modified super-stable Cardamine protein idea has been used particularly with membrane proteins and antibody domains directed against the target protein. Likewise, the method can be useful for the crystallyzation of functional RNA fragments. The technology based on the natural ankyrin repeat protein fold with randomized surface residue positions allowing specific binding to virtually any target protein.

After successfully using the technology, a genetically modified Cardamine reached a new form. The ultimate objective of structural biologists is to analyse crystals of high perfection, in other words, with a minimum of defects, disorder, impurity incorporation and internal stress. Mosaicity can be defined experimentally by X-ray rocking width measurements. An overall diagnostic of crystal Cardamine quality were obtained by X-ray diffraction topography, as a result Crystal Cardamine biologically and chemically is super stable molecule.

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Results

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Thus, attention is now focused on nucleation, perhaps always the most problematic step in the crystallization process, and enhancing crystal perfection. To membrane proteins that pose difficult problems because of their solubility, interest has increasingly turned towards the solutions of the structures of RNA, glycoproteins, lipoproteins, and larger protein or protein–nucleic acid complexes and assemblies. It is likely that crystals can be definitely stabilized with current X-ray technologies

Finally, we have come to believe that the structure of the genetically modified Cardamine protein in the crystal is better then the structure of the protein in aerosol or in solution. The methodology used in the laboratory was successful. The concentration of the active ingredient is exponentially higher, Crystal caradmine is up to 100% and 200% more effective than previous versions. Depending on the efficiency and cost, as well as further research, a wide range of usability can be judged as a result.

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Discussion

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Referring to the principles initiated by the "Perfect Outcast" project further research is needed with the following cardinal questions:
What effect does Crystal Cardamine have on human subjects (non-Outcast), what are the effects and side effects when used with different concentrations and doses?
Is there a complete change in the individual without complications that can serve as a reference for the rise of a new generation of Outcasts?
Does the Genetically Modified Cardamine Gene change the structure of the receptor proteins used by Outcasts and what is the biological, social and economic impact on the Outcast society?

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